Fig. 8
Increased radioresistance of MRC5 CAFs is cancer cell dependent. Micronucleus formation in MRC5 CAFs cocultured for 120 h with MDA-MB-231 or MCF7 breast cancer cells, or MRC5 fibroblasts cocultured with MCF10A non-tumorigenic breast epithelial cells, followed by exposure to 1 Gy of 137Cs γ-rays. The cells were γ-irradiated while in coculture. Fold changes in micronuclei are normalized to respective sham-irradiated (0 Gy) control. Only MRC5 CAFs cocultured with MDA-MB-231 demonstrated significantly increased resistance to ionizing radiation, when compared to control (p < 0.05). The results are representative of three separate experiments