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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: Acquired radioresistance in cancer associated fibroblasts is concomitant with enhanced antioxidant potential and DNA repair capacity

Fig. 4

Chromosomal damage in the CAFs and in their progeny. a Spontaneous chromosomal damage in CAFs. Micronucleus formation in AG1522 CAFs cocultured with MCF7 or MDA-MB-231 breast cancer cells, T98G glioblastoma cells, DU145 prostate carcinoma cells, or H1299 non-small cell lung cancer cells for 120 h without exposure to ionizing radiation. AG1522 CAFs cocultured with H1299 led to a significant increase in micronuclei levels, when compared to AG1522 control (p = 0.01). The results are representative of three separate experiments. b Induction of genomic instability in CAF progeny. Micronucleus formation in AG1522 CAF progeny. AG1522 CAFs were cocultured with themselves or MCF7 breast cancer cells for 5, 24, 48, 72, or 120 h, harvested, and subcultured as a single population for 25 population doublings (PDs). AG1522 progeny cocultured with MCF7 for all time periods demonstrated a significant increase in micronuclei frequency (* = p < 0.05; ** = p < 0.01)

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Cell Communication and Signaling

ISSN: 1478-811X