Fig. 1

Literature-derived L-plastin signalling network and activation state of the output nodes. a A candidate network for the signalling pathways upstream of L-plastin Ser5 phosphorylation was built based on literature information. Green indicates the stimulators used, red the inhibitors, blue the output nodes and yellow the kinases upstream of L-plastin Ser5 phosphorylation. The dashed arrows represent the interactions that are under investigation. b Characterization of the investigated breast cancer cell lines. Determination of the expression of different growth factor receptors in MCF7, SKBR3, HCC38 and BT-20 cells. 50 µg of total cell extract were loaded per sample and β-actin was stained as a loading control. c Example of an immunoblot analysis for L-plastin Ser5 phosphorylation (Ser5-P-LPL) and total L-plastin (LPL). The graph shows the ratio between Ser5-P-L-plastin and L-plastin. The values are represented as means ± SEM of three independent experiments. d The heatmap represents the activation states (scaled between 0 and 1) of the four output nodes in four different cell lines for all the conditions tested