Fig. 3
From: FZD5 prevents epithelial-mesenchymal transition in gastric cancer
TEAD1 coordinates with SNAI2 to inhibit FZD5 transcription. a Binding sites of TEAD1 on FZD5 promoter were shown. b The binding of TEAD1 to FZD5 promoter in HGC27 and MKN45 cells was analyzed by ChIP and real-time PCR. ***P < 0.001, vs IgG. c TEAD1 expression in HGC27 cells was detected by Western blot, and FZD5 expression in HGC27 cells was detected by Western blot and Real-time PCR. ***P < 0.001, vs shCtrl. d Interaction of SNAI2 with TEAD1 in HGC27 cells was analyzed by CoIP. e TEAD1 and FZD5 expression in MKN45 cells was detected by Western blot. f FZD5 expression in MKN45 cells was detected by Western blot and Real-time PCR. ***P < 0.001, vs OE Ctrl; ##P < 0.01, vs OE SNAI2. All experiments were performed in triplicate