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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: miR-1250-5p is a novel tumor suppressive intronic miRNA hypermethylated in non-Hodgkin’s lymphoma: novel targets with impact on ERK signaling and cell migration

Fig. 2

Methylation and expression of AATK/miR-1250 in NHL cell lines. a and b By qRT-PCR, AATK/miR-1250 methylation measured by MSP was associated with lower expression of miR-1250-5p (a) and its host gene AATK (b). c By plotting the expression of AATK against miR-1250-5p, a concordant expression between miR-1250-5p and its host gene AATK was demonstrated. (d–g) In SU-DHL-6 and SUP-T1 cells completely methylated for AATK/miR-1250, treatment with 5-AzadC for 6 days led to AATK/miR-1250 promoter demethylation by MSP (d, e), and associated with re-expression of both intronic miR-1250-5p (f) and its host gene AATK by qRT-PCR (g). Columns represented mean ± 1SD from three experiments in triplicate

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Cell Communication and Signaling

ISSN: 1478-811X