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Fig. 1 | Cell Communication and Signaling

Fig. 1

From: miR-1250-5p is a novel tumor suppressive intronic miRNA hypermethylated in non-Hodgkin’s lymphoma: novel targets with impact on ERK signaling and cell migration

Fig. 1

Methylation of AATK/miR-1250 in healthy normal controls and NHL cell lines. a Schematic diagram showing the relative locations of miR-1250, AATK gene, CpG island, AATK qRT-PCR primer and amplicons of MSP. b Direct sequencing of AATK/miR-1250 M-MSP products from positive control with methylated DNA demonstrated complete bisulfite conversion and MSP specificity. c M- and U-MSP showed that AATK/miR-1250 was unmethylated in healthy normal controls, including normal peripheral blood buffy coats (P1–P10) and normal tonsil tissues (T1–T11). d In NHL cell lines, M- and U-MSP showed that AATK/miR-1250 was completely unmethylated (UU) in GRANTA-519, JEKO-1, MINO, REC-1 and SP-53, and completely methylated (MM) in SU-DHL-6, SU-DHL-16, KARPAS-299, SU-DHL-1 and SUP-T1

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Cell Communication and Signaling

ISSN: 1478-811X