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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: Baicalein inhibits heparin-induced Tau aggregation by initializing non-toxic Tau oligomer formation

Fig. 3

SDS-resistant Tau oligomers-induced by Baicalein. a SDS-PAGE analysis of Tau oligomers. The zero time point did not show presence of any aggregates in control as well as treated samples. At the end of 48 h higher order aggregates (red asterisk) were observed in the 100 μM Baicalein treated sample as compared to control. The 120 h incubated sample showed a decrease in aggregates (green asterisk) in treated samples as compared to control. b The SDS-PAGE quantification for different time points indicate the initial increase followed by the decrease in the aggregates at higher concentrations of Baicalein, which shows significant decrease in 500 µM Baicalein at 48 and 120 h. c The formation of Tau oligomers induced by Baicalein in presence of heparin was also confirmed by immunoblot analysis using the pan-Tau antibody K9JA for total Tau. At 24 h of incubation higher order oligomers were observed in Baicalein treated samples (lane 6, 7) as opposed to control (lane 2). After 48 h of incubation the oligomers population was seen to increase (lane 3, 4) but at 120 h the decrease in the oligomer load was observed (lane 7). (The statistical analysis was carried out by Student’s unpaired t test with respect to Baicalein untreated control. ***p ≤ 0.001, **p ≤ 0.01, *p ≤ 0.05. ns non-significant p value). Further, post hoc analysis was carried by one-way ANOVA and Tukey’s criterion was determined for honestly significant difference (HSD). The data was considered significant if |X–X′|> Tukey’s criterion

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