Fig. 7

eEF2K depletion promotes tumor growth in mice. a shNC- and sheEF2K-expressing A549 cells were implanted (s.c.) into right flanks of Balb/c nude mice as described in Methods. Tumor issues were dissected until 40 days after injection and were photographed. Statistical analysis of the tumor volumes in each group was performed using one-way analysis of variance (Student’s t-test). n = 6, *p < 0.05, **p < 0.01, vs shNC. b Immunohistochemical (IHC) staining of tumor specimens with an anti-PCNA antibody (left). Quantification of PCNA positive cells from tumor specimens (n = 3) from shNC and sheEF2K treated mice (right). The mean and standard deviation are plotted. c Immunoblotting analyses of tumor specimens were performed with the indicated antibodies. d A model of mechanism underlying eEF2K-regulated metabolism and tumor cell proliferation by phosphorylation of PKM2 and regulation of STAT3 phosphorylation and c-Myc expression. The dotted line in figure represents traditional signaling pathway