Fig. 4
eEF2K inhibits PKM2 dimerization. (A, B) A549 cells with or without eEF2K depletion (A) or overexpression (B) were treated with BS3 for 1 h before being lysed. Immunoblotting analyses were performed with the indicated antibodies. (C) A549 cells were cultured in serum-free medium for 12 h, then treated with A484954 for 6 h followed by BS3 treatment for 1 h. Immunoblotting analyses were performed with the indicated antibodies. (D) A549 cells with or without eEF2K depletion were treated with or without EGF for 6 h. The nuclear and cytoplasmic proteins were extracted. Immunoblotting analyses were performed with the indicated antibodies (a). The expressions of PKM2 in nuclear extracts were presented in more obvious manner (b) and the relative levels of the PKM2 to Histone H3 were quantified (c). **P < 0.01, vs no treated EGF. #P < 0.05, vs shNC+EGF