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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: The eEF2 kinase-induced STAT3 inactivation inhibits lung cancer cell proliferation by phosphorylation of PKM2

Fig. 2

eEF2K inhibition increases STAT3 phosphorylation. (A) A549 cells with or without eEF2K depletion were analyzed by immunoblotting analyses with the indicated antibodies. (B) A549 cells with or without eEF2K depletion or overexpression were analyzed by immunoblotting analyses with the indicated antibodies (Ba). The relative phosphorylation levels of the STAT3 to STAT3 were quantified (Bb). (C) eEF2K was immunoprecipitated from lysates of A549 cells. Immunoblotting analyses were performed with the indicated antibodies. (D, E) A549 cell lysates were prepared. Immunoprecipitation and immunoblotting analyses were performed with the indicated antibodies. (F, G) Lysates of A549 cells with or without eEF2K depletion (Fa) or overexpression (Ga) were immunoprecipitated with an anti-PKM2 antibody. Immunoblotting analyses were performed with the indicated antibodies. The relative phosphorylation levels of the STAT3 to PKM2 were quantified (Fb and Gb). **P < 0.01, vs shNC or **P < 0.01, vs Crtl. (H) Lysates of A549 cells with or without eEF2K depletion or overexpression were analyzed by immunoblotting analyses with the indicated antibodies

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