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Fig. 7 | Cell Communication and Signaling

Fig. 7

From: FXR-mediated inhibition of autophagy contributes to FA-induced TG accumulation and accordingly reduces FA-induced lipotoxicity

Fig. 7

Effects of FA and guggulsterone (FXR inhibitor) incubation on FXR-CREB1 pathway and autophagy in yellow catfish hepatocytes at 48 h. a Protein levels of FXR and CREB1. b Co-IP analysis of FXR and CREB1. c Western blot analysis of FXR. d Flow cytometric analysis of LysoTracker, AO and Bodipy, and relative mean fluorescence intensity of MDC, LysoTracker, AO and Bodipy. e Expression of genes involved in autophagy. GS, guggulsterone. FA, oleic and palmitic acid at a ratio of 1:1. Values are means ± SEM (n = 3). Asterisks (∗) indicate significant differences between groups (p < 0.05)

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