Fig. 2

XIAOPI formula inhibits HSPCs recruitment and MDSCs differentiation via inhibiting TAMs/CXCL1. a Flow chart of HSPCs preparation process. Briefly, murine bone marrow cells were flushed out by a syringe under sterile conditions. Lineage⁻/c-Kit⁺ magnetic cell sorting was subsequently conducted. Finally, Sca-1⁺/c-Kit⁺ HSPCs were collected using FACS. b CXCL1 dose-dependently enhanced the proportion of CD11b⁺/Gr-1⁺ cells, facilitated the differentiation of HSPCs towards MDSCs. c-d XIAOPI formula significantly attenuated the movement of HSPCs induced by TAMs-CM, which was relieved by CXCL1 administration. e XIAOPI formula restrained the differentiation of HSPCs to CD11b⁺/Gr-1⁺ MDSCs induced by TAMs-CM and was also reduced by CXCL1 treatment. (The results were obtained from triplicate experiments and were represented as mean values ± SD, *P < 0.05, **P < 0.01)