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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: Exosomal KRAS mutation promotes the formation of tumor-associated neutrophil extracellular traps and causes deterioration of colorectal cancer by inducing IL-8 expression

Fig. 5

Mutant KRAS exosomes induce formation of NETs through upregulation of IL-8; a, mRNA expression of IL-8 in DKs-8 and DKO-1 cells determined by RT-qPCR, * p < 0.05 compared with DKs-8; b, IL-8 protein levels in culture medium after 48 h of DKs-8 and DKO-1 cell culture, analyzed by ELISA, * p < 0.05 compared with DKs-8; c, IL-8 protein levels in culture medium after alternative treatment* p < 0.05 compared with DKs-8 Exos, # p < 0.05 compared with DKO-1 Exos; d, NETs formation and fluorescence quantitative assessment of extracellular DNA fluorescence staining (400×). * p < 0.05 compared with PBS, # p < 0.05 compared to DKO-1 Exos. The measurement data was expressed as mean ± standard deviation. Data comparisons between groups were performed using one-way analysis of variance, followed by Tukey’s post hoc test. The experiment repeated three times

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Cell Communication and Signaling

ISSN: 1478-811X