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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Exosomal KRAS mutation promotes the formation of tumor-associated neutrophil extracellular traps and causes deterioration of colorectal cancer by inducing IL-8 expression

Fig. 2

Exosomes derived from mice with KRAS mutant-CRC mediate IL-8 activation and NET formation; a, protein concentrations of TNF-α, IL-6, IL-8, IL-17 in the serum of APC-WT and APC-KRASG12D mice detected by ELISA; b, NETs in mice, detected by capture ELISA. Values for soluble NET formation are expressed as percentage increase in absorbance above control. Fold increase vs. APC-WT; c, Content of exosomes in serum of APC-WT and APC-KRASG12D mice determined by microBCA protein assay kit at 16th week; d, (Left) Electron microscopy images of APCWT and APC-KRASG12D exosomes, Scale bars = 200 nm; (Right) Size (diameter) distribution of APCWT and APC-KRASG12D exosomes; e, flow cytometry detection of neutrophils binding to PKH67-labeled KRAS mutant-derived exosomes; f, Ratio of neutrophils in colon tissue after administration of exosome of APC-WT and APC-KRASG12D mice; g, Quantification of NET formation after exosome administration of APC-WT and APC-KRASG12D mice; h, IL-8 content in serum of APC-WT and APC-KRASG12D after administration of KRAS mutant exosomes. * p < 0.05, n = 8; The measurement data was expressed as mean ± standard deviation. An unpaired t test was used to compare the two groups. Data analysis at different time points was performed by repeated measurement ANOVA with Bonferroni post hoc test

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