Fig. 2
From: Natural biased signaling of hydroxycarboxylic acid receptor 3 and G protein-coupled receptor 84
Effect of dynasore and sucrose on HCA3 and GPR84 cAMP inhibitory and ERK signaling. a Scheme summarizing all inhibitors and their targets used in the present study. b, c CHO-K1 cells were transiently transfected with HCA3 or GPR84. b 80 μM dynasore reduced cAMP inhibitory signaling of HCA3 upon 3HO and 3HDec and of GPR84 upon C10 but not 3HDec stimulation. Sucrose (0.4 M) significantly inhibited only the 3HDec-induced HCA3-mediated reduction of intracellular cAMP levels. cAMP level of HCA3- or GPR84-transfected cells in absence of agonist is set to 100%, respectively. c The agonist-induced phosphorylation of ERK1/2 was measured in absence and presence of dynasore or sucrose. All agonists induced an increase in pERK/total ERK levels upon stimulation of HCA3 and GPR84, respectively. Dynasore blocked the signal of 100 μM 3HO and 100 μM 3HDec completely and sucrose partially in HCA3–transfected cells. Dynasore did not fully block the signal of 100 μM C10 and 100 μM 3HDec in GPR84-transfected cells. The residual ERK signals of GPR84 in presence of 3HDec and dynasore or sucrose did not differ. pERK/total ERK of HCA3- or GPR84-transfected cells in absence of agonist is set 1. b, c Data is given as mean ± SEM of at least three independent experiments each carried out in triplicates. * P ≤ 0.05; ** P ≤ 0.01