Skip to main content
Fig. 5 | Cell Communication and Signaling

Fig. 5

From: F0F1 ATP synthase regulates extracellular calcium influx in human neutrophils by interacting with Cav2.3 and modulates neutrophil accumulation in the lipopolysaccharide-challenged lung

Fig. 5

F-ATPase/Cav2.3 functional complexes regulate ERK 1/2 phosphorylation and ROS production after fMLP activation of neutrophils. a. A representative western blot is presented to show the effect of F-ATPase/Cav2.3 functional complexes on ERK 1/2 phosphorylation. Neutrophils were pretreated with 400 nM SNX482 and 50 μg/ml oligomycin A in Ca2+-containing HBSS for 30 min, followed by 1 min of 100 nM fMLP activation. Cells in Ca2+-containing HBSS or Ca2+-free HBSS incubated with or without fMLP were used as positive or negative controls, respectively. b. The effect of F-ATPase/Cav2.3 functional complexes on ROS production was assessed by flow cytometry. DHR123-loaded neutrophils were pretreated with 400 nM SNX482 and 50 μg/ml oligomycin A in Ca2+-containing HBSS for 30 min, followed by 30 min of 100 nM fMLP incubation. Cells in Ca2+-containing HBSS or Ca2+-free HBSS incubated with or without fMLP were used as positive or negative controls, respectively

Back to article page