Fig. 4

F-ATPase/Ca_v2.3 complexes modulate extracellular Ca²⁺ influx. (a/left panel) Representative relative fluorescence intensity (RFI, Ex/Em = 488/525 nm) of Fluo-4-loaded neutrophils incubated in Ca²⁺-containing HBSS, with 0.1% DMSO used as a control, 400 nM SNX482 or 50 μg/ml oligomycin A used as an F-ATPase/Ca_v2.3 complex inhibitor and 100 μM thenoyltrifluoroacetone (TTFA) used as a mitochondrial metabolic inhibitor (upper panel). The arrows indicate when 100 nM fMLP was applied. NS/*/*** - P > 0.05/< 0.05/< 0.001 versus the DMSO control (lower panel). The data are presented as the mean ± s.d. of similar triplicate determinations. (b/right panel) Representative RFI of Fluo-4-loaded neutrophils incubated in Ca²⁺-free HBSS with 0.1% DMSO, 400 nM SNX482, 50 μg/ml oligomycin A or 100 μM TTFA (upper panel). The arrows indicate when 100 nM fMLP was applied. NS - P > 0.05 versus the DMSO control (lower panel). The data are presented as the means ± s.d. of similar triplicate determinations