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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Immune response in piglets orally immunized with recombinant Bacillus subtilis expressing the capsid protein of porcine circovirus type 2

Fig. 2

B. subtilis-Cap stimulates phenotypic alteration of BM-DCs and promotes T cells proliferation in vitro. a-fB. subtilis, B. subtilis-Cap, and LPS, were incubated with mouse BM-DCs for 24 h. Expression of CD40, CD80, and MHCII were analyzed by flow cytometry. Untreated BM-DCs served as a negative control. g and h Mixed lymphocyte reaction (MLR) experiments. B. subtilis, B. subtilis-Cap, LPS, or PBS treated BM-DCs were co-cultured with carboxyfluorescein succinimidyl ester (CFSE)-labeled allogeneic T lymphocytes (DC:T cell ratio, 1:1) for another 5 days. Untreated BM-DCs co-cultured with T lymphocytes served as a negative control. T lymphocytes proliferation was evaluated by flow cytometry. Data are presented as means ± SD from three independent experiments. (* 0.01 < p < 0.05, ** p < 0.01)

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Cell Communication and Signaling

ISSN: 1478-811X