Fig. 4
Modulation of the Wnt/β-catenin signaling pathway caused by NAMPT inhibition are NMN-dependent. a TOPFlash assay of LoVo or HCT116 cells treated with 2% DMSO, FK866 (10 nM), or NMN (100 μM) + FK866 (10 nM) for 2 days. The data are presented as the mean ± SD of three independent experiments. Student’s t-test was used for statistical analysis. b The expression of β-catenin and cyclin D1 in LoVo cells with shCtrl + 2% DMSO, shNAMPT + 2% DMSO, or shNAMPT + NMN (100 μM) was analyzed by western blotting. The data are presented as the mean ± SD of three independent experiments. Student’s t-test was used for statistical analysis. c Western blot analysis of whole cell lysates from LoVo cells exposed to 2% DMSO, FK866 (10 nM), or NMN (100 μM) + FK866 (10 nM) for 2 days showed the levels of β-catenin and cyclin D1. The data are presented as the mean ± SD of three independent experiments. Student’s t-test was used for statistical analysis. d Western blot analysis of whole cell lysates from HCT116 cells exposed to 2% DMSO, FK866 (10 nM), or NMN (100 μM) + FK866 (10 nM) for 2 days showed the levels of β-catenin and cyclin D1. The data are presented as the mean ± SD of three independent experiments. Student’s t-test was used for statistical analysis. e The cytoplasmic and nuclear expressions of β-catenin in LoVo cells treated with 2% DMSO, FK866 (10 nM), or NMN (100 μM) + FK866 (10 nM) for 2 days were analyzed by western blotting. The data are presented as the mean ± SD of three independent experiments. Student’s t-test was used for statistical analysis. f The cytoplasmic and nuclear expression of β-catenin in LoVo cells with shCtrl + 2% DMSO, shNAMPT + 2% DMSO, or shNAMPT + NMN (100 μM) was analyzed by western blotting. The data are presented as the mean ± SD of three independent experiments. Student’s t-test was used for statistical analysis. g The expression of β-catenin and cyclin D1 in LoVo cells with shCtrl + 2% DMSO, shNAMPT + 2% DMSO, shCtrl + CHIR99021 (100 ng/mL), or shNAMPT + CHIR99021 (100 ng/mL) was analyzed by western blotting. The data are presented as the mean ± SD of three independent experiments. Student’s t-test was used for statistical analysis. * P < 0.05, ** P < 0.01, and *** P < 0.001 compared with the control group