Fig. 2

Inhibition of NAMPT decreases cell proliferation and induces cell apoptosis in CRC cells. a The protein expression levels of NAMPT in CRC cell lines were shown. b The viability of LoVo or RKO cells treated with 10 nM FK866 or 2% DMSO was analyzed using a CCK-8 assay. The data are presented as the mean ± SD of three independent experiments. Student’s t-test was used for statistical analysis. c The efficiency of NAMPT knockdown in LoVo cells was determined by western blot analysis. The data are presented as the mean ± SD of three independent experiments. Student’s t-test was used for statistical analysis. d The viability of LoVo cells that stably expressed shNAMPT or shCtrl was analyzed using a CCK-8 assay. The data are presented as the mean ± SD of three independent experiments. Student’s t-test was used for statistical analysis. e The efficiency of NAMPT knockdown in RKO cells was determined by western blot analysis. The data are presented as the mean ± SD of three independent experiments. Student’s t-test was used for statistical analysis. f The viability of RKO cells that stably expressed shNAMPT or shCtrl was analyzed using a CCK-8 assay. The data are presented as the mean ± SD of three independent experiments. Student’s t-test was used for statistical analysis. g Representative images of the colony formation assays using LoVo or RKO cells treated with 10 nM FK866 or 2% DMSO. The bar graphs show the quantification of the colony formation assay data. The data are presented as the mean ± SD of three independent experiments. Student’s t-test was used for statistical analysis. h Representative images of the colony formation assays using LoVo or RKO cells with shNAMPT or shCtrl. The bar graphs show the quantification of the colony formation assay data. The data are presented as the mean ± SD of three independent experiments. Student’s t-test was used for statistical analysis. i The efficiency of NAMPT overexpression in SW620 cells was determined by western blot analysis. The data are presented as the mean ± SD of three independent experiments. Student’s t-test was used for statistical analysis. j The viability of SW620 cells that stably overexpressed NAMPT or vector was analyzed using a CCK-8 assay. The data are presented as the mean ± SD of three independent experiments. Student’s t-test was used for statistical analysis. k The expressions of NAMPT and Ki-67 in shNAMPT LoVo, shCtrl LoVo cells, or in LoVo cells treated with DMSO or 10 nM FK866 were analyzed by immunofluorescence staining. The scale bar (white) indicates 20 μm. The quantification of NAMPT and Ki-67 expression are presented as the mean ± SD of three independent experiments. Student’s t-test was used for statistical analysis. l Representative images of the cell apoptosis assays in shNAMPT LoVo, shCtrl LoVo cells, or in LoVo cells treated with DMSO or 10 nM FK866. The bar graphs indicate the percentage of apoptotic cells. The data are presented as the mean ± SD of three independent experiments. Student’s t-test was used for statistical analysis. * P < 0.05, ** P < 0.01, and *** P < 0.001 compared with the control group