Fig. 1

NAMPT expression and prognostic analysis in human colorectal cancer (CRC). a The expression analysis of the proteogenomic study showed NAMPT expression levels in four stages of CRC and adjacent normal tissues. b NAMPT mRNA levels in M0 stage compared with M1 stage using CRC patient tissues. Data were obtained from the Oncomine Cancer Microarray Database using the dataset “Hong Colorectal”. c NAMPT mRNA expression statuses in primary CRC cell line (SW480) and metastatic cell line (SW620) were obtained from GSE1323. SW480 and SW620 are CRC cell lines derived from a primary tumor and a corresponding metastasis lesion from the same patient. d NAMPT mRNA levels in CRC patient tissues compared to nontumorous tissues. Data were obtained from the Oncomine Cancer Microarray Database using the datasets “Gaedcke Colorectal”, “Skrzypczak Colorectal” and “Hong Colorectal”. e Immunohistological (IHC) analysis of NAMPT protein expression in human CRC and corresponding normal tissues using tissue microarrays. Exemplary paired samples are presented. A summarized quantification of the IHC score is depicted. f NAMPT mRNA expression levels (qRT-PCR analysis) in human CRC tissues compared to those in paired normal tissues. g The relative NAMPT protein intensity in the CRC tissues compared to that in the adjacent normal tissues (P < 0.0001, paired t-test). Representative western blotting shows matched CRC and adjacent normal mucosa. h The relapse free survival (RFS) curves were plotted for CRC patients by Kaplan-Meier plotter. * P < 0.05, *** P < 0.001, and **** P < 0.0001 compared with the control group