Fig. 4

YAP depletion results in its activation. a Treatment of ECs with siYAP (iv) resulted in 90% knockdown of YAP (red) in the cytoplasm [15] compared to siCtl treated ECs (i). Nuclei (DAPI blue). vi Detectable amounts of YAP reside within the nucleus (Pink) in YAP knockdown ECs compared to siCtl treatment (iii). Scale bar = 25 μm. b YAP depletion by siRNA results in an increase in its target gene CyR61 as measured by Western blot analysis. Quantification of n = 3 experiments. c YAP depletion by siRNA results in maintenance of p65 in the nucleus and a failure to re-localise back into the cytoplasm after 24 under laminar flow, representative images of one experiment is shown and quantification of the amount of nuclear p65 (NFkB) from n = 3 experiments. p by paired t-test