Fig. 1

YAP is downstream of ARHGAP18. a Deletion of YAP (siYAP) did not affect ARHGAP18 expression under static conditions measured 48 h and 72 h post transfection. Representative Western blot of 3 experiments. b YAP depletion increased activity of RhoC but not RhoA when measured after 48 h of depletion. Results are the pool of 2 individual experiments performed on 2 different EC isolates. c-d YAP expression in ECs lacking ARHGAP18 (siGAP18) is significantly reduced compared to siControl (siCtl) treated ECs under static (c) and 24 h high shear flow (d). Western blots of representative experiment, quantification of mean of 3 experiments. (e) Total and phospho-YAP protein expression in HUVECs infected with either control (EV) or ARHGAP18 (GAP18) containing adenovirus, measured by Western blot, 6 days after infection. β-actin was used as a loading control. Quantification of densitometric ratio for YAP and phopho-YAP presented as the normalised expression of mean of 3 independent HUVEC lines ± SD. P by paired t-test