Fig. 4

TET3 contributed to inducing the stemness of ESCC cells. a FACS was performed to detect the CD133 expression in TET3-negative and TET3-positive group in ESCC patients’ tissues. The plots of a representative ESCC tissue was shown, and the statistical result of a total patients’ data was shown in the upper right corner. b RT-qPCR was performed to TET3 mRNA level in CD133-positive and CD133-positive group in ESCC tissues. c CCK-8 was applied to assess the proliferation ability of ESCC cells with knockdown or overexpression of TET3. d Colony-formation was applied to assess the proliferation ability of ESCC cells with knockdown or overexpression of TET3. e Transwell was employed to assess the migration ability of ESCC cells with knockdown or overexpression of TET3. f Sphere was applied to assess the sphere-formation ability of ESCC cells with knockdown or overexpression of TET3. g CCK-8 was performed to assess the chemoresistance ability of ESCC cells with knockdown or overexpression of TET3. h RT-qPCR was applied to detected stemness-related genes mRNA level in ESCC cells with knockdown or overexpression of TET3. (ns: no significance, *p < 0.05, **p < 0.01, ***p < 0.001)