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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: Epigenetic induction of tumor stemness via the lipopolysaccharide-TET3-HOXB2 signaling axis in esophageal squamous cell carcinoma

Fig. 3

LPS induced TET3 over-expression in ESCC. a The TET protein family mRNA expression in ESCC was analyzed based on TCGA database. b Western blot was performed to detect the TET3 expression in normal esophageal epithelium tissues and ESCC. c FACS was performed to detect the TET3 expression in patients’ normal esophageal epithelium tissues and ESCC tissues. The plots of a representative pair of cancerous and para-cancerous tissues were shown in the left column, and the statistical result of total patients’ data was shown in the right column. d Western blot was performed to detect the TET3 expression in normal esophageal epithelium cell line (HEEC) and ESCC cell lines (EC109, KYSE510, KYSE150 and TE-1). e IF was performed to detect the location of TET3 in ESCC cell lines. f IHC was applied to detect TET3 expression in ESCC tissues from 299 enrolled patients. The typical images of TET3low and TET3high were exhibited. g Kaplan-Meier product limit estimator was applied to draw overall survival (OS) curves of 299 enrolled ESCC patients, compared according to TET3 expression level. h RT-qPCR and Western blot were performed to detect the TET3 expression in ESCC cell lines with the stimulation of LPS. (ns: no significance, *p < 0.05, **p < 0.01, ***p < 0.001)

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