Fig. 8

Abl∆IDR:GFP encodes a stable protein that remains enriched at the cell cortex, like wildtype Abl. a–g Embryos, stages indicated, anterior left. Fixed and stained for Ecad, with the GFP-tagged Abl proteins directly visualized by GFP fluorescence. Abl∆IDR:GFP was expressed in an ablM/Z mutant background except in g, where it was expressed in a wildtype background. Scale bars = 15 µm. a–d. During the extended germband stage, both wildtype Abl:GFP (a, c) and Abl∆IDR:GFP (b, d) have a cytoplasmic pool and are enriched at the cell cortex, as we previously observed is the case for endogenous Abl. e, f Cortical enrichment of both wildtype Abl:GFP and Abl∆IDR:GFP is reduced during dorsal closure. g When expressed in a wildtype background, Abl∆IDR:GFP is enriched in axons in the central nervous system, which are marked by staining of the BP102 antibody. h, i Live imaging of syncytial stage embryos, which were imaged at planes 1, 2, 3 and 4 µm below the vitelline membrane. Wildtype Abl:GFP (h) is clearly cortically enriched but, in contrast, Abl∆IDR:GFP (i) is found throughout the cell. j, k Live imaging of stage 8 embryos during germband extension