Fig. 7

Abl∆IDR does not rescue defects in Ena localization or actin regulation. Leading edge, stage 13–14 embryos, anterior left, dorsal up, stained to visualize Ecad and Ena (a–e) or Ecad and F-actin (f–h). a Wildtype. Ena localizes cortically in both amnioserosal and epidermal cells. Ena is prominently enriched at leading edge tricellular junctions (red arrows), and is enriched at lower levels at tricellular junctions in the lateral epidermis (yellow arrows). Scale bar = 10 µm. b ablM/Z mutant. While Ena remains cortical and is enriched at lateral epidermal tricellular junctions (yellow arrows), uniform Ena enrichment at leading edge tricellular junctions is lost. While some tricellular junctions retain Ena enrichment (red arrows), at others Ena enrichment is reduced (cyan arrows) or Ena is found all along the leading edge (green arrows). c–e Abl∆IDR; ablM/Z mutants. No rescue is observed– Ena remains cortical and is enriched at lateral epidermal tricellular junctions (yellow arrows), but uniform Ena enrichment at leading edge tricellular junctions is lost. Some tricellular junctions retain Ena enrichment (red arrows), while at others Ena enrichment is reduced (cyan arrows) or Ena is found all along the leading edge (green arrows). f Wildtype. Actin is found cortically in all epidermal cells but is enriched in the leading edge actin cable (red arrows). g ablM/Z mutant. While most cells still have actin along the leading edge, actin intensity varies from lower (blue arrows) to much higher than normal (green arrows). Actin is also elevated at tricellular junctions of lateral epidermal cells (yellow arrows). h Abl∆IDR; ablM/Z mutant. Actin alterations are not rescued—while most cells still have actin along the leading edge, actin intensity varies from lower (blue arrows) to much higher than normal (green arrows), and Actin is elevated at many tricellular junctions of lateral epidermal cells (yellow arrows)