Fig. 4

The binding affinity of hPVR mutants with hTIGIT. a, c Parental CHOK1 cells as well as CHOK1 cells overexpressing WT hPVR and mutant hPVR were used in binding assays. The binding of WT and mutant hPVR with hTIGIT-Fc were assessed by flow cytometry using an anti-human Fc antibody. Representative curves of three independent measurements were shown. b, d The binding affinity between hPVR mutants and hTIGIT-Fc at various concentrations from 120 to 1.875 nM. Graphs showed mean ± standard error of the mean (SEM) of three independent experiments. e The binding affinity of hPVR mutants were normalized versus WT hPVR (dashed line) at the concentration of 120 nM, which was defined as relative hTIGIT binding potency (RP) values of the hPVR mutants. *P < 0.05, **P < 0.01 and ***P < 0.001 by Student’s t-test