Fig. 2

Exosomes from metastatic breast cancer cells promoted cancer cell migration and invasion. a Wound-healing cell migration analyses in non-metastatic MCF-7 cells treated with exosomes isolated from conditioned media of metastatic cells (HCC-1937 and MDA-MB-231). b Transwell cell invasion analyses of MCF-7 cells treated with exosomes isolated from HCC-1937 and MDA-MB-231 cells. c, d Comparison of the effect of exosomes from MCF-7 and MDA-MB-231 cells on the cell migration (c) and invasion (d) in HCC-1937 cells. e, f Effect of exosomes from MCF-7 and HCC-1937 on the cell migration and invasion ability of MDA-MB-231 cells examined by wound-healing assay (e) and Transwell invasion assay (f). g, h Effect of exosomes from MDA-MB-231 cells on primary breast cancer cells assessed by wound-healing cell migration (g) and Transwell invasion (h) assays. Each experiment was repeated at least three individual times, and data were presented as mean ± SD. Scale bar: 100 μm, **p < 0.01, ***p < 0.001 compared with control