Table 2 Antibodies
From: Slit/Robo signaling regulates Leydig cell steroidogenesis
Antigen | Company | Catalog number | Dilution factor |
|---|---|---|---|
Antibodies for Western blot analysis | |||
ACTB | Santa Cruz | 47,778 | 1:10,000 |
AKT | Cell signaling | 4691 | 1:1500 |
P-AKT (Ser473) | Cell signaling | 4060 | 1:2000 |
CREB | Cell signaling | 9197 | 1:1000 |
P-CREB (Ser133) | Cell signaling | 9198 | 1:1000 |
GAPDH | Cell signaling | 5174 | 1:10,000 |
mTOR | Cell signaling | 2983 | 1:1000 |
P-mTOR (Ser2448) | Cell signaling | 2971 | 1:1000 |
Antibodies for immunohistochemistry | |||
ROBO1 | Abcam | 7279 | 1:200 |
SLIT1 | Abcam | 129,345 | 1:200 |
SLIT2 | Santa Cruz | 514,499 | 1:50 |
SLIT3 | R&D Systems | MAB3629 | 1:100 |