Table 1 Quantitative RT-qPCR primer sequences
From: Slit/Robo signaling regulates Leydig cell steroidogenesis
Gene | Forward | Reverse |
|---|---|---|
Actb | TCATCACTATTGGCAACGAGC | AGTTTCATGGATGCCACAGG |
Rplp0 | AGATTCGGGATATGCTGTTGG | AAAGCCTGGAAGAAGGAGGTC |
Cyp11a1 | GTGACCTTGCAGAGGTACACTGT | GTGACTCCAGCCTTCAGTTCACA |
Cyp17a1 | CCAGGACCCAAGTGTGTTCT | CCTGATACGAAGCACTTCTCG |
Hsd3b1 | AGCTGCAGACAAAGACCAAGGTGA | GAACACAGGCCTCCAATAGGTTCT |
Lhcgr | AGATGCACAGTGGCACCTTCCAG | ATGACGTGGCGATGAGCGTCT |
Slit1 | CTGCTCCCCGGATATGAACC | TAGCATGCACTCACACCTGG |
Slit2 | AACTTGTACTGCGACTGCCA | TCCTCATCACTGCAGACAAACT |
Slit3 | AGTTGTCTGCCTTCCGACAG | TTTCCATGGAGGGTCAGCAC |
Star | TGTCAAGGAGATCAAGGTCCTG | CGATAGGACCTGGTTGATGAT |
Robo1 | GCTGGCGACATGGGATCATA | AATGTGGCGGCTCTTGAACT |
Robo2 | CTTTGAACGACCCACATTTCTCA | TCTCAGCGTGTAGTCATCTTTGA |