Fig. 6

The expression of Slit and Robo genes is regulated by testosterone. Expression of Slit and Robo genes determined by RT-qPCR in a testes of 2- to 4 month-old wild type mice treated for 24 h with vehicle or 1 mg testosterone (n = 3 per group); b MA10 cells treated for 2 h with vehicle or 1 nM 5α-dihydro-11-keto testosterone (performed three times in triplicate), and c MA10 cells treated for 2 h with vehicle or 1 nM 5α-dihydro-11-keto testosterone ± 20 µM flutamide for 1 h (performed twice in triplicate). Expression of each transcript was normalized to the housekeeping gene Actb or Rplp0, respectively. Data are means ± sem; statistical analysis (Student’s T-test): *p < 0.05; **p < 0.01; ***p < 0.001