Fig. 5

Increased testicular steroidogenesis in Robo1-null mice. a Expression of Star, Cyp11a1 and Cyp17a1 determined by RT-qPCR in primary Leydig cells isolated from Robo1^+/+ vs Robo1Robo1^−/− mice treated for 8 h with vehicle or 10 µg/ml SLIT2. n = 4 samples per group. b Expression of Robo2 determined by RT-qPCR in cultured Leydig cells isolated from Robo1^+/+ and Robo1^−/− mice. n = 4 samples per group. c Expression of Star, Cyp11a1, Cyp17a1, Hsd3b1 and Lhcgr determined by RT-qPCR in testes from four month-old Robo1^+/+ and Robo1^−/− mice. n = 5–7 per group. Expression of each transcript was normalized to the housekeeping gene Rplp0. d Intra-testicular testosterone concentrations corrected to testis weight measured in two- and four month-old Robo1^+/+ and Robo1^−/− mice, and serum LH levels measured in four month-old mice. n = 5–10 per group. Data are means ± sem; statistical analysis (Student’s T-test): *p < 0.05; **p < 0.01; ***p < 0.001; #p < 0.05; ##p < 0.01; ###p < 0.001