Fig. 4

Exogenous SLIT2 alters Leydig cell responsiveness to LH by decreasing Lhcgr expression. a Expression of Lhcgr determined by RT-qPCR in MA10 cells treated for 2, 4, 8 and 24 h with vehicle or 10 µg/ml SLIT2. b Expression of Star, Cyp11a1, Cyp17a1 and Hsd3b1 determined by RT-qPCR in MA10 cells treated with 10 µg/ml SLIT2 for 24 h, ± 50 ng/ml LH for 4 h. c Quantification of total and phospho-CREB protein levels normalized to GAPDH in MA10 cells treated with 10 µg/ml SLIT2 for 24 h, ± 50 ng/ml LH for 30 min. d Expression of Star and Cyp11a1 determined by RT-qPCR in MA10 cells treated with 10 µg/ml SLIT2 for 24 h, ± 10 µM forskolin for 4 h. n = 3–9 samples per group. Expression of each transcript was normalized to the housekeeping gene Rplp0. Data are means ± sem; statistical analysis (Student’s T-test): *p < 0.05; **p < 0.01; ***p < 0.001; #p < 0.05; ##p < 0.01; ###p < 0.001; NS not significant