Fig. 3

Exogenous SLIT2 decreases CREB phosphorylation. a Quantification of total and phosphorylated CREB, AKT and mTOR protein levels normalized to ACTB in MA10 cells treated for 1 h with vehicle or 10 µg/ml SLIT2. Quantification of total and phosphorylated AKT and CREB protein levels normalized to GAPDH, and expression of Star determined by RT-qPCR, in MA10 cells treated with vehicle or b 100 nM wortmannin, or c 20 µg/ml SC79, for 1 h (protein) or 4 h (mRNA). n = 3–9 samples per group. Expression of Star was normalized to the housekeeping gene Rplp0. Data are means ± sem; statistical analysis (Student’s T-test): *p < 0.05; **p < 0.01; ***p < 0.001