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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: PVT1 signals an androgen-dependent transcriptional repression program in prostate cancer cells and a set of the repressed genes predicts high-risk tumors

Fig. 3

Genes de-repressed by PVT1 knockdown in androgen-stimulated LNCaP cells were enriched in tumor suppressor functions. a Gene expression heatmap of the 160 genes (one in each line) that were significantly downregulated by androgen with endogenous PVT1 (CTRL KD, + R1881) and were de-repressed (upregulated) by PVT1 knockdown in androgen-stimulated cells (PVT1 KD, + R1881) (q-value < 0.01, fold-change < 0.5 or fold-change > 2). For each condition indicated at the top, four biological replicates are shown (one in each column); z-score color scale is shown at left. b Gene Ontology terms significantly enriched (Benjamini–Hochberg corrected P < 0.05) with the 160 genes that were de-repressed by PVT1 knockdown in androgen-stimulated cells. c Androgen dose-dependence of FAS gene expression. d Validation by RT-qPCR of the de-repression of FAS expression upon PVT1 knockdown in androgen-starved (blue) or androgen-treated cells (red). e Androgen dose-dependence of NOV gene expression. f Validation by RT-qPCR of the de-repression of NOV expression upon PVT1 knockdown in androgen-starved (blue) or androgen-treated cells (red). T-test, *P < 0.05, **P < 0.01, ***P < 0.005, ****P < 0.001; mean ± s.e.m., three biological replicates

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