Table 1 Used primer sequences for qPCR
From: Chronic inflammation of middle ear cholesteatoma promotes its recurrence via a paracrine mechanism
Primer | Sequence (5′ → 3′) | Size of product (bp) |
|---|---|---|
bFGF | CTGGCTATGAAGGAAGATGGA TGCCCAGTTCGTTTCAGTG | 149 |
Cytokeratin 14 | CTCTAGTGCTGTCACCCAGTT CACAGACACCACGTAGAAGCA | 120 |
Cytokeratin 16 | AAAGCATCCCTGGAGAACAGC CCTCCACACTGCCAATCAGTC | 91 |
Cytokeratin 18 | GACCGCCTGGCCTCTTAC ACCTGGGGTCCCTTCTTCTC | 104 |
Cytokeratin 19 | GAATCGCAGCTTCTGAGACCA CTGGCGATAGCTGTAGGAAGT | 74 |
EGF | GCTGTGTCATTGGATGTGCT TCACCAAAAAGGGACATTGC | 161 |
EREG | TATCACAGTCGTCGGTTCCA AACTCTGGATCCCCTGAGGTA | 108 |
GAPDH | CTGCACCACCAACTGCTTAG GTCTTCTGGGTGGCAGTGAT | 108 |
GM-CSF | TCCTGTGCAACCCAGATTATCA TCATCTGGCCGGTCTCACTC | 116 |
HGF | TGACACGTAGGCTGGAACTG AGTTTGGTGGTCTCCATTGCT | 74 |
IGF-2 | ACGTTCACTCTGTCTCTCCCA CGGGCCAGATGTTGTACTTT | 109 |
IL-1α | TGCCTGAGATACCCAAACC GCCAAGCACACCCAGTAGTC | 145 |
IL-1β | TGTACCTGTCCTGCGTGTTGAAAG CTGGGCAGACTCAAATTCCAGCTT | 149 |
IL-6 | GCAAAGAGGCACTGGCAGAAAACA TTCTGCAGGAACTGGATCAGGACT | 226 |
IL-8 | TCTCTTGGCAGCCTTCCTGATTTC AGTTTTCCTTGGGGTCCAGACAGA | 227 |
KGF | CAGTGGCAGTTGGAATTGTG CCTCCGTTGTGTGTCCATTT | 178 |
Ki-67 | AGTGCTGATGGTTTACAGGGG AGACTCCACGTCTCTTCCCT | 150 |
TGF-β1 | GAGCCCTGGACACCAACTAT GTCCAGGCTCCAAATGTAGG | 167 |
TLR4 | CACAGACTTGCGGGTTCTACATCA TGGACTTCTAAACCAGCCAGACCT | 192 |
TNFα | AAGCCCTGGTATGAGCCCATCTAT AGGGCAATGATCCCAAAGTAGACC | 137 |