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Fig. 7 | Cell Communication and Signaling

Fig. 7

From: Calcium mediated functional interplay between myocardial cells upon laser-induced single-cell injury: an in vitro study of cardiac cell death signaling mechanisms

Fig. 7

Intercellular Ca2+ diffusion at cell–cell junctions in cardiomyocytes. a Time-series images showing Ca2+ propagation along Fluo-4 labeled cardiomyocytes (green) after ablation (refer Additional file 4: movie 4). Ablated spot is indicated in red. The connected cells in the cluster are numbered 1–4 and the connections are marked by white squares. Scale bars: 20 µm. b Immunocytochemical analysis revealed the presence of gap junction protein Cx43 (yellow) at cell–cell contacts (indicated by arrows) between cardiomyocytes from coculture as well as myocytes-enriched culture. The cells were stained for myocyte-specific α-actinin (red), actin filaments (green) and nuclei (blue). Cx43 was absent between myocytes and fibroblasts as well as between fibroblasts in coculture and myocytes-depleted culture. Scale bars: 10 µm

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