Fig. 8
From: PSF functions as a repressor of hypoxia-induced angiogenesis by promoting mitochondrial function
PSF recruited Hakai to inhibit HIF-1α. PSF regulated HIF-1α activation after hypoxia stimulation by Hakai recuitment. Western blotting analysis combined with quantification analysis indicated that PSF overexpression (a, b) reduced whereas PSF knockdown (c, d) the promoted HIF-1α activation levels in nuclear. PSF-Hakai- HIF-1α protein complex formed only after hypoxia induction (e). Hakai siRNA reversed the effects of PSF were confirmed by immunoblotting (f) and statistic analysis (g–i), In PSF-treated lysates of HRMEC transfected with different amounts of Hakai protein, VEGF promoter luciferase activity (j), VEGF protein level (k) and grayscale analysis of VEGF expression were measured (l)