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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: TNFα priming through its interaction with TNFR2 enhances endothelial progenitor cell immunosuppressive effect: new hope for their widespread clinical application

Fig. 4

TNFα priming enhances ECFC immunosuppressive effect. ECFCs were treated with 1 ng/ml of TNFα for 24 h and a randomly assessed for the expression of TNFR2 markers (n = 5). Activated CFSE+CD3+CD25 responder T cells were co-cultured with (b, c) APB-ECFCs (n = 8) and (d, e) CB-ECFCs in different ECFC/T cell ratios (n = 12). Proliferation of CD4+ T cells (b, d) and CD8+ T cells (c, e) was measured by flow cytometry. The first bar represents the unstimulated T cells alone, the second bar represents the anti-CD3/CD28 stimulated T cells alone in 50% RPMI + 50% EGM2 media, while the third bar is the anti-CD3/CD28 stimulated T cells alone in 50% RPMI + 50% EGM2 media + 1 ng/ml TNFα. Data are represented as mean value ± SEM collected from 3 different experiments. One way ANOVA or Student t test analysis was performed to generate P values. ns: non-significant, *P < .05; **P < .01; ***P < .001. Stim: Anti-CD3/CD28 activation Beads

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