Fig. 2
From: The association between RGS4 and choline in cardiac fibrosis
Silencing of RGS4 mitigates cardiac fibrosis in vitro. a Silence of RGS4 by siRNA (si-RGS4) suppresses the abnormal upregulation of collagen I, b collagen III and c α-SMA expression at mRNA level in TGF-β1-stimulated CFs. mRNA level was quantified by qRT-PCR. n = 3. d si-RGS4 suppresses the abnormal upregulation of collagen I, e CTGF and f α-SMA expression at protein level in TGF-β1-stimulated CFs. Protein level was determined by Western blot analysis. n = 4 or n = 5. e Left: representative immunostaining images showing the effects of si-RGS4 on the expression of vimentin in TGF-β1-treated CFs as an indication of fibroblast-myofibroblast transition. Right: statistical results of immunostaining expressed as mean ± SEM. n = 5. f Left: representative immunostaining images showing the effects of si-RGS4 on the expression of α-SMA in TGF-β1-treated CFs as an indication of fibroblast-myofibroblast transition. Right: statistical results of immunostaining. n = 5. g Effect of si-RGS4 on the viability of CFs in the presence of TGF-β1 as measured by MTT assay. n = 6. TGF-β1 + si-NC served as a negative control. *p < 0.05, **p < 0.01, ***p < 0.001 versus Control, #p < 0.05, ##p < 0.01, ###p < 0.001 versus TGF-β1 + si-NC