Fig. 1
From: The association between RGS4 and choline in cardiac fibrosis
RGS4 is upregulated in TGF-β1-treated cardiac fibroblasts (CFs) and in mice with myocardial infarction (MI). a Upregulation of RGS4 expression at both mRNA and b protein levels in TGF-β1-stimulated CFs. mRNA level was quantified by qRT-PCR and protein level was determined by Western blot analysis. n = 3. c Upregulation of RGS4 expression at both mRNA and d protein levels in the heart of mice with MI. n = 4 for mRNA and n = 5 for Western blot. e Verification of RGS4 overexpression in CFs transfected with pcDNA3.1 plasmid containing RGS4 gene. f Increase in collagen I protein level by RGS4 overexpression. n = 5. g Increase in CTGF protein level by RGS4 overexpression. Empty pcDNA3.1 vector serves as a negative control. n = 8 batches of CFs. *p < 0.05, **p < 0.01, ***p < 0.001 versus Control or MI