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Fig. 7 | Cell Communication and Signaling

Fig. 7

From: Histone deacetylase 3 inhibition alleviates type 2 diabetes mellitus-induced endothelial dysfunction via Nrf2

Fig. 7

Knockdown of Nrf2 abolishes HDAC3 inhibition-induced protective effect in vitro. a HUVECs were transduced with si-Nrf2 and a scrambled sequence (scramble) respectively. Protein expression of nuclear accumulation of Nrf2 in HUVECs after transfecting with siRNA assayed by Western blot. b, c The quantitative analysis of nuclear accumulation of Nrf2 and 3-NT immunoblots. d Levels of the oxidative damage marker 3-NT in HUVECs was detected by Western blot. e HUVECs were transduced with si-Nrf2, scrambled sequence combined with si-HDAC3 or not. f, g The quantitative analysis of Nrf2 and Nox4 were evaluated. h Quantification of the TUNEL + cells in (j). i The mRNA level and the quantification of the Nrf2 target gene were evaluated by qRT-PCR. Data are represented as mean ± SEM (n = 5). Significance: *P < 0.05 vs. respective MAN in scrambled HUVECs, #P < 0.05 vs. respective HG-PA in scrambled HUVECs, % P < 0.05 vs. respective HG-PA with si-HDAC3 cultured HUVECs, & P < 0.05 vs. scrambled HUVECs with the same treatment. All above results in graphs (b), (c), (f), (g), (i) were normalized to first group. j TUNEL assay of HUVECs. Scale bars: 100 μm. k Superoxide was determined with DHE. Scale bars: 100 μm. l Capillary-like tube formation was assessed. Scale bars: 300 μm. m Representative confocal images of aortic rings. Scale bars: 400 μm. Values displayed are means ± SEM (n = 5). Significance (h), (n), (o), (p): *P < 0.05 vs. db/db mice, #P < 0./05 vs. RGFP966 treated db/db mice

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