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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: Histone deacetylase 3 inhibition alleviates type 2 diabetes mellitus-induced endothelial dysfunction via Nrf2

Fig. 4

HDAC3 inhibition activates Nrf2 signaling by decreasing Keap1–Nrf2 interaction. a HUVECs were cultured in different condition, and the cell lysates were subjected to immunoprecipitation with Nrf2 antibody, followed by immunoblotting with indicated antibody. b and c The quantitative analysis of (a). d The cell lysates of HUVECs were subjected to immunoprecipitation with Nrf2 antibody, followed by immunoblotting with indicated antibody. e and f The quantitative analysis of (d). g Nrf2 nuclear translocation was determined in fixed cells by immunofluorescent staining in HUVECs. The red area represented Nrf2 and the nucleus was blue. Scale bars: 15 μm. The data are represented as the means ± SEM (n = 4). Significance: *P < 0.05 vs. MAN in scrambled HUVECs, #P < 0.05 vs. HG-PA in scrambled HUVECs, & P < 0.05 vs. HG-PA with si-HDAC3. All above results in graphs were normalized to first group

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