Skip to main content
Fig. 1 | Cell Communication and Signaling

Fig. 1

From: Histone deacetylase 3 inhibition alleviates type 2 diabetes mellitus-induced endothelial dysfunction via Nrf2

Fig. 1

HDAC3 activity and its inhibition are related to endothelial function in T2DM. a, b The immunoblotting and quantitative analysis of HDAC3 protein level relative to GAPDH protein levels in the endothelial cells isolated from db/m mice or db/db mice. Values displayed are means ± SEM (n = 5). c Measurements of deacetylase activity of HDAC3 in different groups of endothelial cells isolated from db/m mice or db/db mice. d Levels of the oxidative damage marker 3-NT in HUVECs were detected by Western blot. e Representative immunofluorescence with CD31 from db/m mice, db/db mice, and RGFP966 or vehicle treated (subcutaneously, 10 mg/kg) db/db mice aorta tissue sections. The red area represented endothelium and the nucleus was blue. Scale bars: 200 μm. f Representative confocal images of oxidative damage marker 3-NT in aortal vascular endothelium. The red area represented endothelium, the green area represents 3-NT positive staining and the nucleus was blue. Scale bars: 40 μm. g Representative confocal images of apoptosis in aortal vascular endothelium. The red area represented endothelium, the green area represents TUNEL positive staining and the nucleus was blue. Scale bars: 40 μm. h The presence of immunofluorescence with CD31 and Ki67 of aortal vascular endothelium, scale bars: 20 μm, the green area represents Ki67 positive staining and the nucleus was blue. i–l Quantification of the number of CD31 positive area staining (e), the number of 3-NT staining (f), TUNEL + cells (g), the proportion of Ki67 positive staining (h). m The quantitative analysis of 3-NT protein immunoblotting, values displayed are means ± SEM (n = 4). Significance (c, i, j, k, l): *P < 0.05 vs. db/m mice; #P < 0.05 vs. db/db mice or vehicle treated db/db mice. Significance (m): *P < 0.05 vs. Con or MAN in scrambled HUVECs; #P < 0.05 vs. HG-PA in scrambled HUVECs. All above results in graphs from western blot were normalized to the first group

Back to article page
\