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Fig. 7 | Cell Communication and Signaling

Fig. 7

From: The COX-2-derived PGE2 autocrine contributes to bradykinin-induced matrix metalloproteinase-9 expression and astrocytic migration via STAT3 signaling

Fig. 7

BK induced astrocytic migration through COX-2/PGE2-mediated MMP-9 expression pathway in RBA cells. a RBA cells were plated on coverslips and grew to confluence, transferred the coverslips to a new 10-cm dish containing serum-free medium for 24 h. Cells were pretreated with CLC (30 μM) for 1 h and then incubated with BK (10 nM) for 48 h. Moreover, cells were pretreated with AG490 (AG, 1 μM), PP1 (1 μM), U0126 (U0, 1 μM, or CBE (0.1 μM) for 1 h and then incubated with PGE2 (10 μM) for 48 h. Phase contrast images of RBA cells were taken at 48 h in response to BK or PGE2, respectively. Representative images are shown for 48 h (insert panel, scale bar = 20 μm, N = 3). The number of BK- or PGE2-induced cell migration at 48 h was counted as described in the Methods. Data are expressed as mean ± SEM of three independent experiments (N = 3). **P < 0.01, as compared with the respective values of cells stimulated with BK only; #P < 0.01, as compared with the respective values of cells stimulated with PGE2 only. The figure represents one of three similar experiments. b Schematic presentation of the role of COX-2/PGE2 system in the BK-induced MMP-9 expression and cell migration. In brain astrocytes (RBA), BK induces COX-2/PGE2-dependent MMP-9 expression via EP-mediated c-Src, Jak2, and ERK1/2 signals resulting in activation of STAT3. The COX-2/PGE2-meditaed MMP-9 expression by BK leads to RBA cell migration

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