Fig. 4
BK induces MMP-9 expression via PGE2-stimulated c-Src-dependent manner in RBA cells. a, b Cells were treated with or without PP1 (1 μM) for 1 h before exposure to 10 nM BK (a) or 10 μM PGE2 (b) for the indicated times. After treatment, the conditioned media were collected and analyzed by gelatin zymorgraphy. c, d Cells were pretreated with or without PP1 (1 μM) for 1 h and then stimulated with BK (c) or PGE2 (d) for 3 and 5 min. The cell lysates were analyzed by Western blot using an antiserum reactive with phospho-c-Src (p–c-Src) antibody and membranes were stripped and re-probed with total GAPDH as a control. Data are expressed as mean ± SEM (a, b bar graph) or mean (c, d) of three independent experiments (N = 3). *P < 0.05; **P < 0.01, as compared with the respective values of cells stimulated with BK (a, c) or PGE2 (b, d) only at the same time. The image represents one of three similar experiments