Fig. 3
PGE2 induces MMP-9 expression via EP receptors in RBA cells. a Time and concentration dependence of PGE2-induced MMP-9 expression, cells were treated with various concentrations of PGE2 (0.1, 1, 10 μM) for the indicated times. After treatment, the conditioned media and cell lysates were collected and analyzed by gelatin zymography (MMP-9) and Western blotting (GAPDH) as described under Methods. b Concentration dependence of PGE2-induced MMP-9 mRNA expression, cells were treated with PGE2 (0, 1, and 10 μM) for 16 h. After treatment, the total RNA were extracted and analyzed by RT-PCR as described under Methods. c Cells were pretreated with or without Sc-19220 (Sc, 3 μM), L798-106 (L798, 3 μM), or GW627368 (GW, 1 μM) for 1 h, and then treated with PGE2 (10 μM) for the indicated times. The conditioned media were collected and analyzed by gelatin zymography. Data are expressed as mean ± SEM of three independent experiments (N = 3). *P < 0.05; **P < 0.01, as compared with the respective values of cells stimulated with vehicle (a, b) and PGE2 (c) only at the same time. The image represents one of three similar experiments