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Fig. 1 | Cell Communication and Signaling

Fig. 1

From: The COX-2-derived PGE2 autocrine contributes to bradykinin-induced matrix metalloproteinase-9 expression and astrocytic migration via STAT3 signaling

Fig. 1

Effect of celecoxib (CLC) on BK-induced MMP-9 up-regulation in RBA cells. a Cells were treated with or without celecoxib (CLC, 30 μM) for 1 h before exposure to 10 nM BK for the indicated times. After treatment, the conditioned media were collected and analyzed by gelatin zymography. b CLC blocked BK-induced MMP-9 mRNA expression in a concentration-dependent manner, cells were pretreated with various concentrations of CLC (1, 10, 30 μM) for 1 h and then stimulated with BK for 16 h. The total RNA was extracted and analyzed by RT-PCR analysis. c Cells were pretreated with CLC (30 μM) for 1 h and then incubated with BK for 16 h. After treatment, the conditioned media were collected and analyzed by PGE2-ELISA kit. d COX-2 is involved in BK-induced MMP-9 expression, cells were transfected with scramble (scra) or COX-2 siRNA and then treated with or without 10 nM BK for the indicated times. The cell lysates were analyzed by Western blot using an antiserum reactive with COX-2 antibody and membranes were stripped and re-probed with total GAPDH as a control (upper panel). The conditioned media were collected and analyzed by gelatin zymography (lower panel). Data are expressed as mean or mean ± SEM (bar graph) of three independent experiments (N = 3). *P < 0.05; **P < 0.01, as compared with the respective values of cells stimulated with BK only at the same time. The image represents one of three similar experiments

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