Skip to main content
Fig. 1 | Cell Communication and Signaling

Fig. 1

From: Anti-Warburg effect by targeting HRD1-PFKP pathway may inhibit breast cancer progression

Fig. 1

Upregulation of HRD1 led to an obvious decrease in robic glycolysis in breast cancer cells. MCF-7 and MDA-MB-231 cells were infected with Ad-GFP or Ad-HRD1 for 48 h. At the end of infection, a glucose uptake was determined by measuring uptake of 2-NBDG using flow cytometry. b Glucose concentration in the medium was measured using the AmplexRed glucose/glucose oxidase assay kit. c Lactate levels in the extracellular medium and the intracellular lactate levels in the cell lysates were measured using a lactate assay kit. d ATP concentration was measured using an ATP assay kit. e Extracellular acidification rate (ECAR) and f oxygen consumption rate (OCR) were measured using a Seahorse XF96 Flux Analyzer. Each point represents the mean ± SD of triplicate determinations

Back to article page